一级特黄AAA大片在线观看,国产一级a毛一级a看免费视频,一级a性色生活片久久无少妇一级婬片免费放,特级丰满少妇一级AAAA爱毛片,综合久久午夜无码鲁丝片久久真人一级毛片,完整观看高清秒播国内外精品资源,欧美成人精品欧美一级乱黄,伊人天天久大香线蕉av色,无毒不卡在线观看无需下载,寡妇高潮一级毛片91免费看｀日韩一区二区

    進(jìn)一步提高血清促甲狀腺激素（TSH）檢測(cè)的敏感性
    增強(qiáng)化學(xué)發(fā)光酶免疫法測(cè)定血清促甲狀腺激素方法的建立及初步應(yīng)用
 通過正交試驗(yàn)，并經(jīng)方差分析，獲得增強(qiáng)發(fā)光酶免疫檢測(cè)法的*實(shí)驗(yàn)條件，將其與TSH-酶聯(lián)免疫吸附測(cè)定（ELISA）試劑盒相結(jié)合，建立TSH的增強(qiáng)化學(xué)發(fā)光酶免疫檢測(cè)法

To improve the sensitivity of measurement of TSH in serum.Methods　Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum 　LIANG Yan，LIN Hanhua，WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University

The authors developed a new enhanced chemiluminescence enzyme immunoassay （ECLIA）, which combined luminol-H2O2-HRP system enhanced by para-iodophenol（PIP） with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
儀器和試劑：Metrohm Herisau E350B-pH計(jì)（瑞士）。魯米諾 （Luminol,Serva） 用pH 10.3、0.05 mol/L 碳酸鹽緩沖液配制成5×10-2 mol/L儲(chǔ)備液放置3天后再以pH 8.2、0.1 mol/L 碳酸氫鈉-氫氧化鈉緩沖液稀釋備用。

將化學(xué)發(fā)光技術(shù)與TSH-ELISA試劑盒相結(jié)合，建立了TSH的增強(qiáng)化學(xué)發(fā)光酶免疫檢測(cè)法